Deuterium-labeled compounds are widely used as research tools in chemistry. Their importance lies in a number of applications, such as:
- proving reaction mechanisms,
- investigation of the pharmacokinetic properties of the given compound,
- internal standards in mass spectrometry,
- compound structure determination in NMR spectroscopy.
Conventional techniques for the synthesis of deuterated compouns utilize D2 gas as a deuterium source. However, there are drawbacks to utilizing deuterium gas on a laboratory scale. Other methods have been employed to overcome the difficulties, such as catalytic H-D exchange reactions between H2 and D2O. These methods are usually time consuming, they do not produce high purity D2 and require high pressure, special catalyst or excess amount of a strong base or acid.
The H-Cube Mini Plus, the H-Cube Pro and the H-Cube Midi continuous flow systems are capable of generating deuterium gas from the electrolysis of D2O, which is readily available in 99,98% purity and is easy to handle.
Deuteration reaction with the H-Cube
Switching from water to deuterium oxide
- Replace existing catalyst with inert TiO2 or blank cartridge. Open the bottom of the water reservoir and remove DI water.
- Push the water tank purge on the info screen and wait 30 seconds to remove the remaining water.
- Close the water reservoir and fill it with 10 mL of heavy water.
- Use the water tank purge on the info screen and wait 1 minute to wash the water line properly with heavy water.
- Open the bottom of the water reservoir and remove the normal-heavy water mixture.
- Push the water tank purge on the info screen and wait 30 seconds to remove the remaining normal-heavy water mixture.
- Close the water reservoir and fill it with heavy water.
- If it is important to remove the traces of the normal water, repeat the points 4-7.
- Start using the system with heavy water.
- Place the new cartridge into the H-Cube and run heavy water through for a simulated run prior to the planned reaction.